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Most likely, your lighting conditions subtly changed over time, or you may have bumped your camera. Either way, hitting Recalibrate will fix the issue 99.9% of the time.
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If you are seeing this a lot, you might want to check to make sure neither of these two things are happening:
Back-lighting is causing the shooter, or something or someone else, to cast shadows over the targets. Those shadows cause changing light conditions. You will want to remove the source of the back-lighting, or use additional fill lighting to eliminate the shadows.
Natural lighting from the sun is dominating the light in the room. The sun moves throughout the day, butts move in front of the sun, and light levels in the room and on your targets change accordingly. You will want to take steps to eliminate this source of changing light, like closing curtains, or even moving to a different room.
And obviously: That the targets and webcam are not wobbling/moving around, and that the shooters are not moving between the webcam and the targets.
If you are in a situation where you simply can't control the lighting, such as an event or show, you can also try adjusting the sensitivity setting in Target Options. Each target zone has its own setting. You'll want to adjust them to be less sensitive.
KEEP IN MIND: Adjusting this will make LASR less sensitive to ALL light, including laser impacts, and adjusting it may cause shots to go undetected.